DNA FRAGMENT CAPABLE OF CODING NON-A, NON-B HEPATITIS VIRUS STRUCTURAL PROTEIN

Japanese Patent JP3195799

B2

PURPOSE: To provide the subject new DNA fragment coding a polypeptide useful for diagnosis of non-A, non-B hepatitis excellent in accuracy of judgment.
CONSTITUTION: This invention is a DNA fragment obtained by collecting non-A, non-B hepatitis virus RNA directly from the plasma of a non-A, non-B hepatitis patient and modifying the collected RNA according to the genetic engineering method, containing a base sequence capable of coding a polypeptide constituting non-A, non-B hepatitis virus structural protein and having an amino acid sequence of, e.g. the formula. From the fresh plasma of a Japanese chronic non-A, non-B hepatitis patient infected only with group II, all the RNAs including non-A, non-B hepatitis virus RNA are collected using ELISA method and PCR method and cDNA is synthesized according to random primer method. The resultant cDNA is introduced in λ phage so as to construct a cDNA library. The obtained cDNA library is subjected to immunoscreening using the above- mentioned plasma of the hepatitis patient and cDNA library constructed from plural patient plasmas is screened by using the resultant DNA fragment as a probe, thus affording the objective cDNA specific to the above-mentioned hepatitis.

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JP3688290	Hepatitis C virus test

Noboru Maki
Kenjiro Yamaguchi
Toyoshima Ayumi
Ohara Doho

JP41384490A

August 06, 2001

December 20, 1990

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Advanced Life Science Institute Co., Ltd.

C07K14/18; C07K14/00; C07K14/02; C07K14/10; C12N1/21; C12N15/09; C12N15/51; C12P21/02; G01N33/53; G01N33/576; C12R1/19; (IPC1-7): C12N15/09; C07K14/18; G01N33/53; G01N33/576

Ｂｉｏｃｈｅｍ．Ｂｉｏｐｈｙｓ．Ｒｅｓ．Ｃｏｍｍｕｎ．，１７０（１９９０）ｐ．１０２１−１０２５
Ｊａｐａｎ．Ｊ．Ｅｘｐ．Ｍｅｄ．，６０（１９９０）ｐ．１６７−１７７

Yoshio Kawaguchi (2 outside)

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