To provide a DNA probe chip for performing the hybridization of polynucleotide in a sample solution of DNA, RNA or the like with the probe DNA fixed to a substrate at a high speed in a high yield, and a hybridization method.

DNA probes are fixed to the surfaces of a large number of the pillars 7 provided in a probe fixing region. When a sample solution is introduced, an electric field is controlled so that a target nucleotide is introduced into the valley parts of pillars 7. Subsequently, the electric field is reversed to increase the concentration of the target nucleotide on the side of the probe fixing region.