Title:
DNA POLYMERASE COMPOSITION FOR NUCLEIC ACID AMPLIFICATION
Document Type and Number:
Japanese Patent JP3463780
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a heat-stable DNA polymerase composition excellent in amplification efficiency of nucleic acid.
SOLUTION: This composition comprises a modified heat-stable DNA polymerase (first DNA polymerase) having 0-5% 3'-5'-exonuclease activity in comparison with a DNA polymerase having 3'5' exonuclease activity before modification and a modified heat-stable DNA polymerase (second DNA polymerase) having 100-6% 3'5' exonuclease activity in comparison with the heat-stable DNA polymerase having 3'-5'-exonuclease activity and the heat-stable DNA polymerase having 3'5'-exonuclease activity before the modification. The first DNA polymerase and the second DNA polymerase have a DNA synthesis rate of at least 30 bases/second and heat stability capable of maintaining ≥60% remaining activity at pH8.8 at 95°C for 6 hours treatment. A long-term nucleic acid can be amplified by using the composition.
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Inventors:
Shusuke Komatsubara
Masao Kitabayashi
Hideki Uemura
Fumikiyo Kawakami
Yoshihisa Kawamura
Masahiro Takagi
Tadayuki Imanaka
Masao Kitabayashi
Hideki Uemura
Fumikiyo Kawakami
Yoshihisa Kawamura
Masahiro Takagi
Tadayuki Imanaka
Application Number:
JP20044696A
Publication Date:
November 05, 2003
Filing Date:
July 30, 1996
Export Citation:
Assignee:
TOYO BOSEKI KABUSHIKI KAISHA
International Classes:
C12N15/09; C07H21/04; C12N9/12; C12Q1/68; (IPC1-7): C12N15/09; C12N9/12; C12Q1/68
Domestic Patent References:
| JP838198A | ||||
| JP7298879A |
Other References:
【文献】国際公開95/016028(WO,A1)
【文献】国際公開92/009689(WO,A1)
【文献】国際公開92/009689(WO,A1)