Title:
DNA SEGMENT GIVING HIGH FREQUENCY FORM INDUCTION OF RECOMBINANT DNA VECTOR
Document Type and Number:
Japanese Patent JPS63230091
Kind Code:
A
Abstract:
A segment (hft) of bacteriophage FP43 DNA cloned into plasmids pIJ702 or pMT660 mediates high frequency transduction of these plasmids by FP43 in Streptomyces griseofuscus. Lysates of FP43 prepared on S. griseofuscus containing the plasmids with the hft segment also transduce many other Streptomyces species, including several species that restrict plaque-formation by FP43 and at least two species that produce restriction endonucleases that cut the plasmid DNA. Transduction efficiencies in different species are influenced by the addition of anti-FP43 antiserum to the transduction plates, the temperature for cell growth before transduction, the multiplicity of infection, and the host on which the transducing lysate is prepared. Transducing lysates prepared on S. griseofuscus are also able to mediate plasmid transfer to species of Streptoverticillium, Chainia, and Saccharopolyspora.
Inventors:
RICHIYAADO HENRII BARUTSU
MAAGARETSUTO AN MAKUHENNII
MAAGARETSUTO AN MAKUHENNII
Application Number:
JP4853588A
Publication Date:
September 26, 1988
Filing Date:
March 01, 1988
Export Citation:
Assignee:
LILLY CO ELI
International Classes:
C12N15/76; C12N15/09; (IPC1-7): C12N15/00
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)
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