Title:
E. COLI EXPRESSION VECTOR
Document Type and Number:
Japanese Patent JP2007185197
Kind Code:
A
Abstract:
To provide a fed-batch fermentation process which uses special E. coli host/vector systems for the purpose of efficiently forming recombinant proteins, in particular recombinant antibody molecules, preferably antibody fragments such as miniantibodies.
An E. coli expression vector which is suitable for expressing exogenous proteins under high cell density fermentation conditions is constituted by using at least (i) an upstream terminator sequence and a downstream terminator sequence, (ii) lac promoter/operator system, (iii) a T7g10 Shine/Dalgarno sequence, (iv) pelB or ompA signal sequence, and (v) the sequence of an exogenous gene.
Inventors:
STRITTMATTER WOLFGANG
MATZKU SIEGFRIED
RIESENBERG DIETER
HORN UWE
KNUEPFER UWE
KUJAU MARIAN
WENDEROTH ROLF
PLUECKTHUN ANDREAS
KREBBER ANKE
MATZKU SIEGFRIED
RIESENBERG DIETER
HORN UWE
KNUEPFER UWE
KUJAU MARIAN
WENDEROTH ROLF
PLUECKTHUN ANDREAS
KREBBER ANKE
Application Number:
JP2007109443A
Publication Date:
July 26, 2007
Filing Date:
April 18, 2007
Export Citation:
Assignee:
MERCK PATENT GMBH
International Classes:
C12N15/09; C07K16/00; C07K16/28; C12N1/21; C12N15/70; C12N15/72; C12P21/00; C12P21/02; C12R1/19
Attorney, Agent or Firm:
Akio Miyazaki
Ishibashi Masayuki
Masaaki Ogata
Ishibashi Masayuki
Masaaki Ogata