To provide a new enzyme for phosphorylating 22 position of ecdysteroid and its gene and to provide a method or the like for simply and inexpensively producing a phosphoric acid conjugate of the ecdysteroid by using the enzyme.
The inventors elucidated that ecdysteroid-22-phosphorylated enzyme is isolated and refined from ovary of a silkworm pupa by using column chromatography and ecdysteroid is practically phosphorylated by using the enzyme and ecdysteroid-22-phosphoric acid can be synthesized thereby. Furthermore, the inventors determined gene sequence (base sequence of cDNA) encoding the enzyme and whole amino acid sequence of the protein. The enzyme of the present invention is utilized for control of insect pests by inactivating ecdysis hormone and the enzyme enables production of various useful ecdysteroid phosphoric acid conjugates utilized as reagents for researches and further enables utilization to development, etc., of medicines and cosmetics.
IEGI KATSUNORI
MAEDA SEIKO
OHIRA TAKESHI
MARCY N WILDER
MITA KAZUHIDE
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