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Title:
ELECTROCHEMILUMINESCENT ASSAY
Document Type and Number:
Japanese Patent JPH07267972
Kind Code:
A
Abstract:

PURPOSE: To provide a novel compound useful for detection of enterotoxins and germs as an electrochemiluminescent labeling reagent.

CONSTITUTION: This compound is represented by the formula: X-Y-Z [wherein X is a nucleotide, antibody, analyzing substance or the like; Y is a linker group having C atoms; and Z is formula I or II (wherein R is an anion, and (n) is an integer) or the like]. The compound is obtained by reacting chemical species emitting electrochemiluminescence such as bis(2,2'-bipyridine) [4-(4-methyl-2,2'- bipyridine-4'-yl)butyric-acid]ruthenium(II)dihexafluorophosphate, and an X compound such as digoxigenin in the presence of 1,3-dicyclohexylcarbodiimide in pyridine anhydride.


Inventors:
Massey, Richard J.
Powell, Michael J.
Mied, Paul A.
Feng, Peter
Della, Ciana Leopoldo
Dressick, Walter J.
Poonian, Mohindar S.
Application Number:
JP1994000275174
Publication Date:
October 17, 1995
Filing Date:
November 09, 1994
Export Citation:
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Assignee:
IGEN INC
International Classes:
G01N33/532; C07D213/22; C07D213/26; C07D213/30; C07D213/48; C07D213/54; C07D213/55; C07D213/73; C07D213/78; C07D401/06; C07D401/14; C07D405/06; C07D405/14; C07D473/08; C07F15/00; C07H19/073; C07H21/00; C07H21/04; C07J9/00; C07J17/00; C07J41/00; C07K1/113; C07K14/15; C07K14/575; C07K14/705; C07K14/76; C07K16/00; C09K11/06; C09K11/07; C12Q1/04; C12Q1/68; G01N21/76; G01N21/78; G01N27/26; G01N27/416; G01N33/533; G01N33/543; G01N33/569; G01N33/58; G01N33/76; (IPC1-7): C07F15/00; C07D213/26; C07D213/48; C07D213/54; C07D213/73; C07D405/06; C07K14/575; G01N21/78; G01N33/533; G01N33/58
Attorney, Agent or Firm:
浅村 皓 (外2名)