Title:
エレクトロポレーション用電極及び外来物質導入装置
Document Type and Number:
Japanese Patent JP6660057
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a technology for attaining a stable reciprocating motion of a droplet in a droplet electroporation method, the technology allowing the effective introduction of a foreign substance to be executed in a stable manner.SOLUTION: According to the present invention, an electroporation electrode electroporates a cell in a droplet formed in an insulating liquid to allow a foreign substance to be introduced into the cell. In the electroporation electrode comprises a structure, (A) a core electrode and a surrounding electrode are included, (B) the core electrode is an electrode member served as a first polar electrode disposed inside the surrounding electrode with a gap from the surrounding electrode, and (C) the surrounding electrode is an electrode member served as a second polar electrode circumferentially provided around the core electrode with a gap from the core electrode.SELECTED DRAWING: Figure 5
Inventors:
Rika Numano
Kazuki Nakao
Kojiro Matsumoto
Kazuki Nakao
Kojiro Matsumoto
Application Number:
JP2015242198A
Publication Date:
March 04, 2020
Filing Date:
December 11, 2015
Export Citation:
Assignee:
Toyohashi University of Technology
RIKEN
Neppagene Co., Ltd.
RIKEN
Neppagene Co., Ltd.
International Classes:
C12M1/00; C12M1/42; C12N13/00; C12N15/87
Domestic Patent References:
| JP2007520994A |
Foreign References:
| WO2014092164A1 |
Other References:
浅田淳, et al.,電界中の油中液滴を用いる遺伝子導入法の開発,静電気学会講演論文集2011,2011年,p.155-158
林良茂ら,同軸円筒型電極による活性汚泥の脱水分離,水質汚濁研究,1992年,Vol.15, No.9,p.600-611
KURITA, H. et al.,Novel Parallelizaed Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor,PLOS ONE,2015年12月 9日,p.1-15
林良茂ら,同軸円筒型電極による活性汚泥の脱水分離,水質汚濁研究,1992年,Vol.15, No.9,p.600-611
KURITA, H. et al.,Novel Parallelizaed Electroporation by Electrostatic Manipulation of a Water-in-Oil Droplet as a Microreactor,PLOS ONE,2015年12月 9日,p.1-15
Attorney, Agent or Firm:
Youichi Tanabe