Title:
【発明の名称】選択性の向上した電気泳動ゲル
Document Type and Number:
Japanese Patent JP2000517431
Kind Code:
A
Abstract:
Electrophoresis gels of enhanced selectivity are produced by adding a preformed polymer to a polymerization solution containing at least one monomer and at least one cross-linker. The polymer must be of such a chemical composition and molecular weight that its presence during free-radical polymerization results in a new topology of gel polymers, so that under the influence of an electric field, such as during electrophoresis, the passage of long DNA molecules through the gel is retarded proportionally more than the passage of the short ones. The polymer needs to be added at specific ratio to the monomer and the cross-linker, and the enhanced selectivity is achievable only within a certain range of ratios of polymer(s), monomer(s) and cross linker(s). That range is dependent on all three essential gel components, the polymer, the monomer and the cross-linker. Some gels possessing enhanced selectivity were able to resolve one base pair differences between DNA fragments in the 100 bp range with the gel length of less than 10 cm.
Inventors:
Cozlick, swing
Application Number:
JP51241698A
Publication Date:
December 26, 2000
Filing Date:
September 05, 1997
Export Citation:
Assignee:
Guest Elkrom Scientific AG
International Classes:
C07K1/26; C08F220/56; C08F251/00; C08F265/00; G01N27/447; (IPC1-7): G01N27/447
Attorney, Agent or Firm:
Yoshiki Hasegawa (2 outside)