Title:
標的ポリヌクレオチドを検出するためのコード化反応およびデコード反応
Document Type and Number:
Japanese Patent JP2007530048
Kind Code:
A
Abstract:
The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.
Inventors:
Lao, Kai Kin
Reed, Mark Tee.
Reed, Mark Tee.
Application Number:
JP2007505187A
Publication Date:
November 01, 2007
Filing Date:
March 24, 2005
Export Citation:
Assignee:
Appraera Corporation
International Classes:
C12Q1/68; C12N15/09; G01N33/53
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita