Title:
エンドＭ変異体、及びＮ結合型糖鎖含有化合物又はＮ結合型糖鎖含有蛋白質の製造方法
Document Type and Number:
Japanese Patent JP6830576
Kind Code:
B2
Abstract:
An embodiment of the present invention provides: an endo-M variant having an amino acid sequence in which the amino acid residue at position 251 of the amino acid sequence represented by SEQ ID NO: 1 is asparagines or alanine, and having an activity for catalyzing a hydrolysis reaction represented by the following reaction formula (1) (X is a group derived from carbohydrates, and Y is a monovalent substituent); or an endo-M variant having an amino acid sequence in which the amino acid residue at position 251 of the amino acid sequence represented by SEQ ID NO: 1 is asparagines or alanine, having a modified amino acid sequence having at least 80% homology to the amino acid sequence via deletion, addition or substitution of one or a plurality of amino acid residues other than the amino acid residue at position 251 of the amino acid sequence represented by SEQ ID. NO: 1, and having an activity for catalyzing the hydrolysis reaction represented by the following reaction formula (1).
Inventors:
Kenji Yamamoto
Norihiko Kato
Norihiko Kato
Application Number:
JP2017502530A
Publication Date:
February 17, 2021
Filing Date:
February 26, 2016
Export Citation:
Assignee:
Tokyo Chemical Industry Co., Ltd.
International Classes:
C12N9/42; C12P21/00; C12P21/02
Other References:
梅川碧里 ほか著,糸状菌由来エンドグリコシダーゼ(Endo-M)の新規なグライコシンターゼ様変異体の創製と糖鎖複合体の効率的合成への応用,応用糖質科学,2013年 5月20日,Vol.3, No.2,pp.143-150,ISSN 2185-6427, 特に要旨、第144頁左欄第26-42行
加藤紀彦 ほか著,バイオ医薬品創出に向けたEndo-M基質特異性の改変,日本農芸化学会2015年度大会講演要旨集,公益社団法人日本農芸化学会,2015年 3月 5日,p.2D34P11,ISSN 2186-7976, 全文
加藤紀彦 ほか著,バイオ医薬品創出に向けたEndo-M基質特異性の改変,日本農芸化学会2015年度大会講演要旨集,公益社団法人日本農芸化学会,2015年 3月 5日,p.2D34P11,ISSN 2186-7976, 全文
Attorney, Agent or Firm:
Atsushi Nakajima
Kato Kazunori
Hiroshi Fukuda
Kato Kazunori
Hiroshi Fukuda