Title:
ENRICHING DIHALOGENASE-CONTAINING BACTERIUM, METHOD FOR SEPARATING AND CULTURING THE BACTERIUM AND PAPER PRODUCT CONTAINING RESIDUE OF BACTERIUM
Document Type and Number:
Japanese Patent JP3752141
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for enriching a bacterium containing a dehalogenating enzyme so as to increase individual group of a bacterium to a level effective for dehalogenating a nitrogen-free organohalogen compound present in a polymerization reacrion product, separating the bacterium and successively culturing the separated bacterium, and to obtain a paper product composed of a nitrogen-containing cationic polymer and the residue of bacterium.
SOLUTION: This method comprises (A) adding an environmental sample containing plural solid groups of bacteria to a nitrogen-containing polymer and a nitrogen-free organohalogen compound, (B) incubating the mixed solid groups of bacteria, (C) separating a dihalogenase-containing bacterium from the incubated bacterium solid groups and (D) culturing the dihalogenase-containing bacterium solid group until reaching at least 5×107 cells per milliliter. The paper product comprises (A) a nitrogen- containing cationic polymer and (B) the residue of bacterium present on the maximum of about 100 g based on 1 ton of dried paper product in which the bacterium contains an enzyme capable of dehalogenating the nitrogen free orgenohalogen compound without substantially changing the polymer.
Inventors:
David Jay Hardman
Alan Bull
Brian M Stubbs
Alan Bull
Brian M Stubbs
Application Number:
JP2000306611A
Publication Date:
March 08, 2006
Filing Date:
April 24, 1992
Export Citation:
Assignee:
HERCULES INCORPORATED
International Classes:
C12N1/00; C08G69/48; C12N9/14; C08G73/02; C12N1/02; C12N1/20; C12P1/00; C12P1/04; C12P5/00; C12P7/04; C12S99/00; D21H11/20; D21H17/00; D21H17/18; D21H17/21; D21H17/45; D21H17/55; D21H21/14; D21H21/20; D21H27/00; C12R1/01; C12R1/06; C12R1/13; C12R1/15; C12R1/38; (IPC1-7): C12N1/00; C12N1/02; D21H17/18; D21H17/21; D21H17/45; //C12N9/14; (C12N1/00; C12R1:01); (C12N1/00; C12R1:06); (C12N1/00; C12R1:13); (C12N1/00; C12R1:15)
Domestic Patent References:
| JP2170825A | ||||
| JP53147804A |
Other References:
Journal of Bacteriology, 1991 Jan, Vol.173, No.1, pp.124-129
FEMS Microbiology Letters, 1991 June, Vol.81, No.2, pp.171-176
Agric.Biol.Chem.,1990, Vol.54, No.12, p.3185-3190
FEMS Microbiology Letters, 1991 June, Vol.81, No.2, pp.171-176
Agric.Biol.Chem.,1990, Vol.54, No.12, p.3185-3190
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Hiroaki Noya
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Hiroaki Noya