Title:
ENZYME-IMMUNITY FLUORESCENT TESTING METHOD OF ANTI-TREPONEMAANTIBODY
Document Type and Number:
Japanese Patent JPS60205366
Kind Code:
A
Abstract:
A method for the determination of anti-treponemal antibody in a test sample comprises contacting a substrate for the anti-treponemal antibody with sample; treating the contacted substrate with labeled antihuman lg antibody selected from (a) a mixture comprising enzyme labeled antihuman Ig antibody and fluorescent labeled antihuman lg antibody, (b) antihuman Ig antibody labeled with an enzyme and a fluorescent label, and (c) fluorescent labeled antihuman Ig antibody to which enzyme labeled antibody against the animal species from which the antibody used in the fluorescent labeled antibody was derived is added subsequently, and (d) enzyme labeled antihuman Ig antibody to which fluorescent labeled antibody against the animal species from which the antibody used in the enzyme labeled antibody was derived is subsequently added; determining the enzyme activity of the treated substrate; and determining the immunofluorescent patterns in substrates exhibiting enzyme activity. The method is useful for the rapid screening of anti-treponemal antibodies for the diagnosis of syphilis.
Inventors:
SUTEFUAN AARU KOOTESU
UORUTAA ERU BINDAA
UORUTAA ERU BINDAA
Application Number:
JP3778885A
Publication Date:
October 16, 1985
Filing Date:
February 28, 1985
Export Citation:
Assignee:
HOECHST CO AMERICAN
International Classes:
C12P21/08; C12Q1/28; G01N33/571; G01N33/569; (IPC1-7): A61K39/00; C12P21/00; C12Q1/00; G01N33/569
Domestic Patent References:
| JPS58108458A | 1983-06-28 | |||
| JPS5630824A | 1981-03-28 |
Attorney, Agent or Firm:
Takehiko Saito (1 outside)