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Title:
ESTERASE STRUCTURAL GENE, ITS TRANSFORMED STRAIN AND PRODUCTION OF VINEGAR USING THE STRUCTURAL GENE
Document Type and Number:
Japanese Patent JP3908352
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To obtain the subject new gene comprising the DNA of a structural gene coding for an esterase protein having a specific amino acid sequence and capable of giving recombinant Acetobacter, etc., for producing excellent vinegar containing high amounts of ethyl acetate and isoamyl acetate.
SOLUTION: This invention relates to a new DNA of an esterase structural gene coding for a protein expressed by an amino acid sequence expressed by the formula and vinegar containing high amounts of ethyl acetate and isoamyl acetate and excellent in flavor can be produced by using recombinant Acetobacter introduced with fragments of the gene. This esterase structural gene is obtained by preparing a genome library of Acetobacter, Acetobacter pasteurianus, with Escherichia coli as a host, replicating the genome library on filtration paper, lysing the bacteria by immersing in a bacteriolytic solution, selecting colonies, which are changed to red color by a color developing method, as a clone strain of the esterase gene and harvesting the gene.


Inventors:
Yasuhiro Kashima
Iijima Masumi
Kenji Tayama
Akiko Okamoto
Koizumi Komichi
Uzaka Shigezo
Yanagita Fujiharu
Application Number:
JP26825997A
Publication Date:
April 25, 2007
Filing Date:
September 16, 1997
Export Citation:
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Assignee:
Nakano Vinegar Co., Ltd.
International Classes:
C12N15/09; C12J1/04; C12N1/21; C12N15/00; C12P7/54; C12R1/02; C12R1/19; (IPC1-7): C12N15/09; C12J1/04; C12N1/21; C12P7/54; //(C12N15/09; C12R1:02); (C12J1/04; C12R1:19); (C12N1/21; C12R1:19); (C12P7/54; C12R1:19)
Other References:
鹿島康浩(外 5名),酢酸菌のエステラーゼに関する研究,日本醸造協会誌,Vol.91, No.9,P.659 (1996)
Attorney, Agent or Firm:
Toda parent man