Title:
トランスジェニック動物乳房組織における活性ヒト第IX因子の発現
Document Type and Number:
Japanese Patent JP4936568
Kind Code:
B2
Abstract:
Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5'-untranslated and 3'-untranslated region, which is substituted with a 5-' and 3'-end of the mouse whey acidic protein gene. In vitro cell cultures of cells explanted from the transgenic mammal of the invention and methods of producing Factor IX from such said culture and methods of treating hemophilia B are also described.
Inventors:
Jiyon el jiyonson
William H. Bellander
William N Drawan
Henrik Rubon
William H. Bellander
William N Drawan
Henrik Rubon
Application Number:
JP2009256131A
Publication Date:
May 23, 2012
Filing Date:
November 09, 2009
Export Citation:
Assignee:
American red cross
International Classes:
A01K67/027; A61K35/20; A61K38/43; A61K38/46; A61K48/00; A61P7/00; A61P7/04; A61P43/00; C07K14/435; C07K14/745; C12N5/10; C12N9/64; C12N15/09; C12N15/85; C12P21/02; A61K38/00
Domestic Patent References:
| JP4365487A | ||||
| JP8501936A |
Foreign References:
| WO1995030000A1 | ||||
| WO1995022249A1 | ||||
| WO1988000239A1 |
Other References:
Proc. Natl. Acad. Sci. USA,1995年,vol. 92,10899-10903
Endocrionol.,1996年,vol. 137,321-330
Bio/Technology,1989年,vol. 7,487-492
EMBO J.,1984年,vol. 3,1053-1060
Endocrionol.,1996年,vol. 137,321-330
Bio/Technology,1989年,vol. 7,487-492
EMBO J.,1984年,vol. 3,1053-1060
Attorney, Agent or Firm:
Yoshio Kawaguchi
Chihiro Watanabe
Katsuma Osaki
Mitsuaki Tsubokura
Chihiro Watanabe
Katsuma Osaki
Mitsuaki Tsubokura