EXPRESSION PLASMID AIMING AT EXPRESSION OF RAT HEPATIC CYTOCHROME P-450MC GENE IN ESCHERICHIA COLI

Title:

EXPRESSION PLASMID AIMING AT EXPRESSION OF RAT HEPATIC CYTOCHROME P-450MC GENE IN ESCHERICHIA COLI

Document Type and Number:

Japanese Patent JPS615783

Kind Code:

A

Abstract:

PURPOSE: To obtain a plasmid for expression in Escherichia coli, having a high hydroxylating activity, capable of expressing cytochrome P-450MC having wide substrate specificity in the Escherichia coli, and applicable to oxidation reaction processes on an industrial level and oxidative removal of organic compounds in industrial waste water, etc.

CONSTITUTION: A plasmid pMG1 obtained by linking a structural gene of cytochrome P-450MC isolated from a recombinant plasmid pAU157 (producible by the method described in Nud. Acids. Res. 12, P2929W2938) having the whole coding region of rat hepatic cytochrome P-450MC protein to a tac promoter which is a powerful promoter of Escherichia coli. The tac promoter is constituted of -35 region of trp promoter and -10 region of lacUV5 promoter.

Inventors:

OOEDA KENJI
NAKAMURA KEIKO
OOKAWA HIDEO

Application Number:

JP12295284A

Publication Date:

January 11, 1986

Filing Date:

June 16, 1984

Export Citation:

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Assignee:

KOGYO GIJUTSUIN

International Classes:

C12N15/09; C07H21/04; C12N1/21; C12N9/02; C12R1/19; (IPC1-7): C07H21/04; C12N15/00; C12R1/19

Other References:

NUCLEIC ACIDS RESEARCH=1984
J.BIOCHEM=1983

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