EXPRESSION AND PURIFICATION OF RECOMBINANT SOLUBLE TISSUE FACTOR

Title:

EXPRESSION AND PURIFICATION OF RECOMBINANT SOLUBLE TISSUE FACTOR

Document Type and Number:

Japanese Patent JP2003238600

Kind Code:

A

Abstract:

To provide a method and a means for enabling a purified protein, especially a blood clotting protein to be isolated by a single chromatographic step.

A fusion protein contains an epitope bound by a monoclonal antibody nominated as HPC-4, deposited with the American Type Culture Collection, Rockville, MD, on Nov. 2, 1988, and assigned as ATCC No. HB 9892 in the presence of calcium, and a protein to be isolated by the bond of the fusion protein with the HPC-4 antibody. The epitope and the protein to be isolated are separated at a specific protease cleavage site.

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Inventors:

REZAIE ALIREZA
ESMON CHARLES T
MORRISSEY JAMES H

Application Number:

JP2003027695A

Publication Date:

August 27, 2003

Filing Date:

December 29, 1992

Export Citation:

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Assignee:

OKLAHOMA MED RES FOUND

International Classes:

C07K1/107; C07K14/47; C12N15/09; C07K14/745; C07K16/40; C07K19/00; C12N9/64; C12P21/02; G01N33/68; G01N33/86; A61K38/00; C12R1/91; (IPC1-7): C07K19/00

Attorney, Agent or Firm:

Shusaku Yamamoto

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