Title:
細胞からの抽出
Document Type and Number:
Japanese Patent JP5886940
Kind Code:
B2
Abstract:
A method for releasing the content of the periplasmic space of bacterial cells is provided, which comprises incubating the bacterial cells in a solution containing styrene maleic acid copolymer (SMA). Also provided is a method of preparing a substantially pure sample of recombinant polypeptide. The methods find application in the recovery of materials, such as proteins, from bacterial cells.
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Inventors:
Duffon, Timothy
Tylinis-Thomas, Owen Robert
Tylinis-Thomas, Owen Robert
Application Number:
JP2014509806A
Publication Date:
March 16, 2016
Filing Date:
May 09, 2012
Export Citation:
Assignee:
The University of Birmingham
International Classes:
C12N1/20; C12N1/21; C12P21/02
Foreign References:
| WO2011004158A1 | ||||
| US4595658 |
Other References:
J. Am. Chem. Soc., 2009, Vol.131, pp.7484-7485
Attorney, Agent or Firm:
Keiichiro Saikyo