Title:
酵素の抽出および精製
Document Type and Number:
Japanese Patent JP2006525788
Kind Code:
A
Abstract:
A process for purifying a phosphodiesterase 1 (PDE-1) from a cell including heating an extract of a cell formed from a solution including at least on divalent cation, to increase the specific activity of PDE-1 in the extract.
Inventors:
Patane, Michael
Application Number:
JP2004571747A
Publication Date:
November 16, 2006
Filing Date:
December 04, 2003
Export Citation:
Assignee:
Protech Research Pty Limited
International Classes:
C12N9/16; C07H19/10; C07H19/20; C12N5/04; C12N15/55; C12P19/30
Domestic Patent References:
| JPH04504649A | 1992-08-20 |
Other References:
JPN6009051638, Ｂｉｏｃｈｅｍ． Ｍｏｌ． Ｂｉｏｌ． Ｉｎｔ．， ｖｏｌ．２９， ｐ．１０９５−１１０２ （１９９３）
JPN6009051637, Ｅｎｚｙｍｅ Ｍｉｃｒｏｂ． Ｔｅｃｈｎｏｌ．， ｖｏｌ．１１， ｐ．４４４−４５１ （１９８９）
JPN6009051636, 酵素ハンドブック 第４４９頁
JPN6009051637, Ｅｎｚｙｍｅ Ｍｉｃｒｏｂ． Ｔｅｃｈｎｏｌ．， ｖｏｌ．１１， ｐ．４４４−４５１ （１９８９）
JPN6009051636, 酵素ハンドブック 第４４９頁
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita