Title:
Fc receptor-based affinity chromatography
Document Type and Number:
Japanese Patent JP6360232
Kind Code:
B2
Abstract:
Herein is reported the use of an immobilized non-covalent complex of a neonatal Fc receptor (FcRn) and beta-2-microglobulin (b2m) as affinity chromotography ligand in general and, for example, for the determination of the in vivo half-live of an antibody by determining the ratio of the retention times of the antibody and a reference antibody.
Inventors:
Falkenstein Robert
Hertenberger Fubert
Rueger Petra
Schlotauer Tillman
Hertenberger Fubert
Rueger Petra
Schlotauer Tillman
Application Number:
JP2017104130A
Publication Date:
July 18, 2018
Filing Date:
May 26, 2017
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
B01J20/282; C07K1/22; C07K16/00; C40B30/04; G01N30/88; G01N33/68
Domestic Patent References:
| JP2008504002A | ||||
| JP2010520748A | ||||
| JP2009148265A |
Foreign References:
| WO2011106272A1 |
Other References:
HUBER ANDREW H.,Crystallization and Stoichiometry of Binding of a Complex between a Rat Intestinal Fc Receptor and Fc,JOURNAL OF MOLECULAR BIOLOGY,ACADEMIC PRESS,1993年 4月 5日,V230 N3,P1077-1083
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto