To provide a film forming method capable of forming a film, containing protein with superior film forming accuracy and capable of restraining or preventing reduction in the physiological activities of the protein in the film, a substrate with the film equipped with the film formed by the film forming method, and a sensor with high reliability, and to provide a liquid composition, capable of preventing or restraining reduction of the physiological activities of the protein included in the formed film, and capable of forming the film with superior film forming accuracy.

The film forming method for forming a detection film, including a detection substance for detecting a detection target on one surface side of the substrate, is provided with a first process for preparing the liquid composition containing the protein and a polyether-based compound having weight average molecular weight of 2,000 or lower; a second process for forming a liquid coated film, by supplying the liquid composition to the one surface side with the usage of a droplet jetting method; and a third process for forming the detection film by drying the liquid coated film.

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