PURPOSE: To obtain the titled fine particles useful for clinical measurements using immunochemical reactions, etc., capable of immobilizing an antigen, antibody, etc. to the surfaces of them firmly by a chemical bond, by irradiating a specific monomer with light rays of ionizing radiation at a lower temperature ≤ room temperature.
CONSTITUTION: A polymerizable monomer (e.g., crotonaldehyde, etc.) containing an aldehyde group and a polymerizable double bond is irradiated with light rays or ionizing radiation in a low-temperature state ≤ room temperature, preferably at 5W-100°C, so that the monomer is polymerized, to give the desired particles having an aldehyde group on the surfaces and 0.5W20 micron particle diameters. The exposed dose of the light rays, etc. is preferably ≤1×106R. In order to immobilizing an antigen, antibody, etc. to the particles, the particles are washed with water, etc., and brought into contact with the antigen, antibody, etc. under a given condition.
EFFECT: Having high sensitivity, capable of detecting even a very small amount of a substance.
USE: Clinical examinations using a receptor reaction, lectin reaction, etc.
KAETSU ISAO
SUZUKI MIEKO
ADACHI SHIYOUICHI
NIHON KOUTAI KENKYUSHO KK
| JPS58140256A | 1983-08-19 | |||
| JPS50113591A | 1975-09-05 |