PURPOSE: To prepare stable and water-insoluble lactase having high hydrolyzing ability of lactose, by fixing lactase derived from Aspergillus oryzae on an ampholytic ion exchange resin of phenol formaldehyde of microporous type.
CONSTITUTION: Lactase derived from Aspergillus oryzae is adsorbed on an amphoytic ion exchange resin of phenol formaldehyde of microporous type having a specific surface not less than 1m2/g, total pore volume of micropores with pore diameters 100W2,000 not less than 0.1cc/g, an anion exchange capacity based on amino or substituted amino group not less than 1meg/g and a cation exchange capacity based on carboxymethyl group not less than 0.5meg/g as a carrier in an aqueous solution in pH 4.0W6.5, the fixed lactase is treated with an aqueous solution of glutaraldehyde in pH 3.5W7.0, or glutaraldehyde is linked to the carrier and reacted with lactase.
YAMAMOTO HIDEFUMI
KOUNO EMIKO
NABESHIMA NARIYASU
MITSUTA MASARU
NAGASE TSUNEYUKI
| JPS5411908A | 1979-01-29 | |||
| JPS54119084A | 1979-09-14 |
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