Title:
フラップエンドヌクレアーゼ変異体。
Document Type and Number:
Japanese Patent JP5110448
Kind Code:
B2
Abstract:
A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.
Inventors:
Eriko Matsui
Ikuo Matsui
Ikuo Matsui
Application Number:
JP2010096197A
Publication Date:
December 26, 2012
Filing Date:
April 19, 2010
Export Citation:
Assignee:
National Institute of Advanced Industrial Science and Technology
International Classes:
C12N15/09; C12N9/16; C12Q1/42
Other References:
The Journal of Biological Chemistry,2002年,Vol.277, No.40,p.37840-37847
J.Mol.Biol.,2003年,Vol.328,p.537-554
The Journal of Biological Chemistry,2004年 6月,Vol.279, No.23,p.24394-24402
The Journal of Biological Chemistry,1996年,Vol.271, No.16,p.9173-9176
J.Mol.Biol.,2003年,Vol.328,p.537-554
The Journal of Biological Chemistry,2004年 6月,Vol.279, No.23,p.24394-24402
The Journal of Biological Chemistry,1996年,Vol.271, No.16,p.9173-9176