To provide a fluorescence microscope capable of realizing the scanning of a sample in a direction Z without changing a relative distance between a sample and an objective optical system.
The fluorescence microscope is equipped with a fixed mirror arranged between a wavelength separating element and a scanning unit and deflecting luminous flux from the wavelength separating element, a movable mirror deflecting the deflected luminous flux in a specified direction further, a condensing optical system condensing the luminous flux deflected by the movable mirror and generating specified curvature-of-field aberration in accordance with the rotational angle of the movable mirror, and a reflection mirror reflecting the luminous flux condensed in the condensing optical system. Then, the sample is scanned in the direction Z without changing the relative distance between the sample and the objective optical system by an optical system arranged on the front side focal position of the condensing optical system and converting parallel beams made incident from the movable mirror to the reflected light of focused luminous flux or divergent luminous flux in accordance with the rotational angle of the movable mirror.
Masatake Shiga
Masakazu Aoyama
Suzuki Mitsuyoshi
Tadao Takashiba
Kunio Ueda