Title:
蛍光ヌクレオチド及びそれを用いた標識法
Document Type and Number:
Japanese Patent JP4676654
Kind Code:
B2
Abstract:
Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X-Y-Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1 to R4 represent an alkyl group; V1 to V6 represent a hydrogen atom or a substituent; L1 to L7 represent a methine group; W represents an oxygen atom, a sulfur atom, -C(R3)(R4)- or -N(R5)- wherein R3 to R5 represents an alkyl group; Q represents a nitrogen atom or -C(V7)- wherein V7 represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1 or R2
More Like This:
Inventors:
Junji Nishigaki
Takeshi Nakamura
Kazuya Takeuchi
Hiroko Inomata
Masayoshi Kojima
Takeshi Nakamura
Kazuya Takeuchi
Hiroko Inomata
Masayoshi Kojima
Application Number:
JP2001219211A
Publication Date:
April 27, 2011
Filing Date:
July 19, 2001
Export Citation:
Assignee:
FUJIFILM Corporation
International Classes:
C07H19/10; G01N33/53; C07D471/04; C07D498/04; C07D513/04; C07H19/20; C07H21/00; C07H21/02; C07H21/04; C09B23/00; C12N15/09; C12Q1/68; G01N33/533; G01N33/566
Domestic Patent References:
| JP9124599A | ||||
| JP4080747A | ||||
| JP1280750A | ||||
| JP2001163895A | ||||
| JP9127115A | ||||
| JP8059689A | ||||
| JP7027768A | ||||
| JP6122696A |
Foreign References:
| WO2001021624A1 | ||||
| WO2002026891A1 | ||||
| GB870753B | ||||
| WO1999040223A1 | ||||
| WO1999037717A1 | ||||
| WO1998058942A1 | ||||
| WO1998030992A1 |
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes