FRAMEWORK-SHUFFLING OF ANTIBODIES

Japanese Patent JP2013013425

A

To produce a rapid and simple combinatorial library of antibodies (with or without constant regions) that can be easily screened for their immunospecificity for an antigen of interest, as well as their immunogenicity in an organism of interest.

The present invention relates to methods of reengineering or reshaping antibodies to reduce the immunogenicity of the antibodies, while maintaining the immunospecificity of the antibodies for an antigen. In particular, the present invention provides methods of producing antibodies immunospecific for an antigen by synthesizing a combinatorial library comprising complementarity determining regions (CDRs) from a donor antibody fused in frame to framework regions from a sub-bank of framework regions.

WU HERREN
DALL ACQUA WILLIAM
DAMSCHRODER MELISSA

JP2012236592A

January 24, 2013

October 26, 2012

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MEDIMMUNE LLC

C12P21/08; C12N15/09

WO2003042247A2	2003-05-22
WO1999053038A2	1999-10-21
EP0699755A2	1996-03-06
WO1998052976A1	1998-11-26
WO2000034317A2	2000-06-15
US20050048617A1	2005-03-03

JPN6011045071; Ｈｙｂｒｉｄｏｍａ　ａｎｄ　ｈｙｂｒｉｄｏｍｉｃｓ．　２００３，　Ｖｏｌ．２２，　Ｎｏ．４，　ｐ．２４５-２５７
JPN6011047009; Ｍｅｔｈｏｄｓ　ｉｎ　ｍｏｌｅｃｕｌａｒ　ｂｉｏｌｏｇｙ．　２００３，　Ｖｏｌ．２０７，　ｐ．１９７-２１２
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Yusuke Hiraki
Satoshi Fujita
Eiichi Arai
Natsuo Tanaka

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