Title:
FREE LIGAND MEASURING METHOD
Document Type and Number:
Japanese Patent JP3418429
Kind Code:
B
Abstract:
PURPOSE: To allow more accurate measurement of the concentration of free ligand initially existing in a mixture of an object to be measured and a protein having a bond therewith under dynamic equilibrium.
CONSTITUTION: When an object to be measured, i.e., a ligand, and a protein having a bond therewith coexist in a specimen and bonding equilibrium is present between them, only the free ligand initially existing in the specimen is measured specifically. In this method, a substance having a bond with the object to be measured is added into the specimen while being fixed to an insoluble carrier thus finishing the reaction for measurement before it is affected by the object being fed from a bonded ligand.
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Inventors:
Takada, Masato
Kishida, Mariko
Kishida, Mariko
Application Number:
JP1993000198192
Publication Date:
April 11, 2003
Filing Date:
August 10, 1993
Export Citation:
Assignee:
MITSUBISHI CHEM CORP
International Classes:
G01N33/53; G01N33/543; G01N33/53; G01N33/543; (IPC1-7): G01N33/53; G01N33/543