To provide a fuel injection device for a motorcycle, improving air amount responsiveness and suppressing transfer of heat and vibration from an engine to fuel injectors.

A throttle body 17 having a main throttle valve 40 opened/closed by throttle operation and an auxiliary throttle valve 41 of which opening/closing drive is electronically controlled is provided. The throttle body 17 is connected with a cylinder head 23 comprising the engine 16 via an intake pipe 31, and an insulator 38 formed of an elastic body is arranged between the intake pipe 31 and the cylinder head 23. A first fuel injector 43 is fitted to the intake pipe 31, and a second fuel injector 45 is fitted to the throttle body 17.