融合タンパク質、物質製造方法、ベクター、形質転換細胞、空気入りタイヤの製造方法及びゴム製品の製造方法

Japanese Patent JP6923166

B2

Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, the first and second amino acid sequences being fused via a linker sequence (third amino acid sequence) while maintaining the enzymatic activity of the second amino acid sequence.

Haruhiko Yamaguchi
Yukino Inoue
Seiji Takahashi
Toru Nakayama
Satoshi Yamashita
Yuzuru Tozawa

JP2019131427A

August 18, 2021

July 16, 2019

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Sumitomo Rubber Industries, Ltd.
Tohoku University
Kanazawa University
Saitama University

C12N15/62; B29D30/06; C07K14/415; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12N15/29; C12N15/54; C12N15/63; C12P5/00

JP2004527227A
JP2015097523A
JP2015136296A

WO1996021029A1
WO2017002818A1

Horn, P. J. et al.,"Identification of a new class of lipid droplet-associated proteins in plants",Plant Physiol.,2013年,Vol. 162,pp. 1926-1936

Atomi International Patent Office

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