Title:
GLP-1受容体アゴニスト・バイオアッセイ
Document Type and Number:
Japanese Patent JP2011523052
Kind Code:
A
Abstract:
Provided herein are cell-based bioassays for measuring GLP-1 receptor agonist activity of test compounds, such as GLP-1 receptor agonist compounds. Exemplary GLP-I1 receptor agonist compounds include exendins, exendin analogs, GLP-1(7-37), and GLP-1(7-37) analogs. The bioassays are useful for quantitatively determining cAMP generated in samples containing GLP-1 receptor agonist compounds (e.g., exenatide) and 6-23 (clone 6) cells having GLP-1 receptors, whereby the amount of cAMP generated can be correlated to the GLP-1 receptor agonist activity of the GLP1 receptor agonist compounds (e.g., exenatide). Suitable cell-based bioassays include enzyme-linked immunosorbent assays and homogeneous time-resolved fluorescence assays.
Inventors:
Michelis Nikolaou
Frederick Charles Bancroft
John Patrick Helich
Aun Nine Ruwin
Swati gupta
Frederick Charles Bancroft
John Patrick Helich
Aun Nine Ruwin
Swati gupta
Application Number:
JP2011510592A
Publication Date:
August 04, 2011
Filing Date:
May 15, 2009
Export Citation:
Assignee:
AMYLIN PHARMACEUTICALS, INC.
International Classes:
G01N33/50; G01N21/64; G01N21/78; G01N33/15
Foreign References:
| WO2008005527A2 | 2008-01-10 |
Other References:
JPN6013025518; ＴＥＣＡＮ アプリケーションノート , 2007
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Tsuyoshi Sato
Masaki Yano
Keiji Sakata
Hiroshi Yamazaki
Tsuyoshi Sato
Masaki Yano
Keiji Sakata