Title:
コドン翻訳効率に基づく遺伝子発現系
Document Type and Number:
Japanese Patent JP2006500927
Kind Code:
A
Abstract:
The present invention discloses a method for modulating the production of a protein from a polynucleotide in a CHO cell by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower translation efficiency in the CHO cell than the codon it replaces, or by introducing into the CHO cell a polynucleotide that codes for an iso-tRNA which limits the rate of production of the polypeptide and which corresponds to a codon of the first polynucleotide. The present invention also discloses the use of a protein-encoding polynucleotide whose codon composition has been modified for enhanced production of the protein in CHO cells.
Inventors:
Fraser Ian Hector
Application Number:
JP2004534865A
Publication Date:
January 12, 2006
Filing Date:
September 15, 2003
Export Citation:
Assignee:
Rohm and Haas Electronic Materials LLC
International Classes:
C12N15/09; A01H1/00; C07K14/025; C07K14/47; C12N5/06; C12N5/10; C12N7/00; C12N15/18; C12N15/28; C12N15/37; C12N15/67; C12P21/02
Domestic Patent References:
| JPH07504203A | 1995-05-11 | |||
| JPH04503154A | 1992-06-11 |
Foreign References:
| WO2000042215A1 | 2000-07-20 | |||
| WO1999002694A1 | 1999-01-21 | |||
| WO1997026333A1 | 1997-07-24 | |||
| WO1992005248A1 | 1992-04-02 |
Other References:
JPN6009056743, Ｔｒａｎｓｇｅｎｉｃ Ｒｅｓ．, 1999, Ｖｏｌ．８， Ｎｏ．５, ｐｐ．３７１−３８１
JPN6009056747, Ｊ． Ｖｉｒｏｌ．, 1999, Ｖｏｌ．７３， Ｎｏ．６, ｐｐ．４９７２−４９８２
JPN6009056747, Ｊ． Ｖｉｒｏｌ．, 1999, Ｖｏｌ．７３， Ｎｏ．６, ｐｐ．４９７２−４９８２
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi
Koichi Niimi