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Patent Searching and Data


Title:
コドン翻訳効率に基づく遺伝子発現系
Document Type and Number:
Japanese Patent JP2006500927
Kind Code:
A
Abstract:
The present invention discloses a method for modulating the production of a protein from a polynucleotide in a CHO cell by replacing at least one codon of the polynucleotide with a synonymous codon that has a higher or lower translation efficiency in the CHO cell than the codon it replaces, or by introducing into the CHO cell a polynucleotide that codes for an iso-tRNA which limits the rate of production of the polypeptide and which corresponds to a codon of the first polynucleotide. The present invention also discloses the use of a protein-encoding polynucleotide whose codon composition has been modified for enhanced production of the protein in CHO cells.

Inventors:
Fraser Ian Hector
Application Number:
JP2004534865A
Publication Date:
January 12, 2006
Filing Date:
September 15, 2003
Export Citation:
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Assignee:
Rohm and Haas Electronic Materials LLC
International Classes:
C12N15/09; A01H1/00; C07K14/025; C07K14/47; C12N5/06; C12N5/10; C12N7/00; C12N15/18; C12N15/28; C12N15/37; C12N15/67; C12P21/02
Domestic Patent References:
JPH07504203A1995-05-11
JPH04503154A1992-06-11
Foreign References:
WO2000042215A12000-07-20
WO1999002694A11999-01-21
WO1997026333A11997-07-24
WO1992005248A11992-04-02
Other References:
JPN6009056743, Transgenic Res., 1999, Vol.8, No.5, pp.371−381
JPN6009056747, J. Virol., 1999, Vol.73, No.6, pp.4972−4982
Attorney, Agent or Firm:
Hatsushi Shimizu
Koichi Niimi