To provide a new gene participating in acetic acid tolerance of an acetic acid bacterium and to provide a method for improving the acetic acid tolerance of a microorganism, especially the acetic acid bacterium using the gene and a method for efficiently producing vinegar at a higher acetic acid concentration using the acetic acid bacterium having improved acetic acid tolerance.
The new gene having functions to improve the acetic acid tolerance from the practical acetic acid bacterium belonging to the genus Gluconacetobacter is cloned by a method for obtaining a gene proliferating even in a culture medium at an acetic acid concentration in which proliferation cannot usually be carried out from a chromosomic DNA library of the acetic acid bacterium. In a transformant in which the gene is transferred into the acetic acid bacterium, the acetic acid tolerance is remarkably improved. When the transformant is aerobically cultured in the presence of ethanol, the proliferation induction phase can be shortened and even the proliferation rate can be improved. Furthermore, the final ultimate acetic acid concentration can remarkably be improved.
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FURUSATO MASAKO