Title:
遺伝子およびポリペプチド配列
Document Type and Number:
Japanese Patent JP4597668
Kind Code:
B2
Abstract:
The present invention provides a method for improving a leader sequence by insertion of a pentapeptide motif comprising -X 1 -X 2 -X 3 -X 4 -X 5 - and a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif:
-X 1 -X 2 -X 3 -X 4 -X 5 -
where X 1 is phenylalanine, tryptophan, or tyrosine, X 2 is isoleucine, leucine, valine, alanine or methionine, X 3 is leucine, valine, alanine or methionine, X 4 is serine or threonine and X 5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.
Inventors:
Darrell, sleep
Application Number:
JP2004522368A
Publication Date:
December 15, 2010
Filing Date:
July 23, 2003
Export Citation:
Assignee:
Novozymes By Offer Madeco Actiezel Scab
International Classes:
C12N15/09; C07K14/76; C07K14/765; C07K14/79; C07K19/00; C12N1/15; C12N1/19; C12N15/62; C12N15/81; C12P21/02; A61K38/00; C07K7/06; C07K7/08
Domestic Patent References:
| JP3103179A | ||||
| JP11502112A |
Foreign References:
| WO1993018181A1 |
Other References:
Biochemistry, vol. 28, pages 923-930 (1989)
Collins F.S et al.,Accession:AAE02154 [GI:5942622], Definition: Sequence 2 from patent US 5859195.
Neuron, vol. 9, pages 209-216 (1992)
J. Biochem., vol. 110, pages 103-110 (1991)
FEMS Microbiol. Rev., vol. 24, pages 45-66 (Jan. 2000)
Proc. Natl. Acad. Sci. USA, vol. 79, pages 71-75 (1982)
BIO/TECHNOLOGY, vol. 8, pages 217-221 (1990)
Collins F.S et al.,Accession:AAE02154 [GI:5942622], Definition: Sequence 2 from patent US 5859195.
Neuron, vol. 9, pages 209-216 (1992)
J. Biochem., vol. 110, pages 103-110 (1991)
FEMS Microbiol. Rev., vol. 24, pages 45-66 (Jan. 2000)
Proc. Natl. Acad. Sci. USA, vol. 79, pages 71-75 (1982)
BIO/TECHNOLOGY, vol. 8, pages 217-221 (1990)
Attorney, Agent or Firm:
Hirohito Katsunuma
Yukitaka Nakamura
Noritaka Yokota
Takeyasu Ito
Yukitaka Nakamura
Noritaka Yokota
Takeyasu Ito