Title:
GENETICALLY MODIFIED PHAGE AND USE THEREOF
Document Type and Number:
Japanese Patent JP2017158551
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method using a genetically modified phage that does not restore its lytic property when the phase is used to produce a biomolecule, thereby avoiding a culture medium from being contaminated with an infectious phage or preventing the phase from being inadvertently dissolved during growth or protein production.SOLUTION: According to the present invention, there is provided a genetically modified phase in which, based on the fact that ral gene and N gene are related to a lysogenic state and deletion of a DNA fragment containing coding sequences of ral gene and N gene leads to the death of the host cell, at least one gene selected from Int gene, Xis gene, R gene, S gene, Q gene, and Rz gene is inactivated. There is also provided a process for producing a biomolecule using a host cell containing the genetically modified phage. The genetically modified phase comprises a T7 expression system as an expression system and is defined as a lambda phage, 434 phage, phi80 phage, phi81 phage, HK97 phage, or P21 phage, or one from P 11 phage to P 53 phage.SELECTED DRAWING: Figure 1
Inventors:
CEDRIC SZPIRER
Application Number:
JP2017044126A
Publication Date:
September 14, 2017
Filing Date:
March 08, 2017
Export Citation:
Assignee:
DELPHI GENETICS
International Classes:
C12N7/01; C12N1/21; C12P21/02
Domestic Patent References:
| JP2004528005A | 2004-09-16 |
Other References:
BIOTECHNOL.PROG., vol. vol.9, JPN6016008129, 1993, pages 153 - 159
JOURNAL OF BACTERIOLOGY, vol. 184, no. 18, JPN6016008132, 2002, pages 5200 - 5203
JOURNAL OF BACTERIOLOGY, vol. 184, no. 18, JPN6016008132, 2002, pages 5200 - 5203
Attorney, Agent or Firm:
Ryoichi Takaoka
Nao Oda
Kaoru Takahashi
Nao Oda
Kaoru Takahashi