To provide a germicidal composition or a germicidal disinfectant composition for dilution for adequately using chlorhexidine gluconate against important bacterial strains of nosocomial infectious diseases.

The germicidal composition comprises chlorhexidine gluconate for disinfecting important bacterial strains of nosocomial infectious diseases (1) in a chlorhexidine gluconate concentration of 0.02 w/v% to 4 w/v% and (2) in a disinfecting time of 30 sec to 60 min as an active ingredient.