GLUTAMINE-AUXOTROPHIC HUMAN CELL CAPABLE OF PRODUCING PROTEIN AND CAPABLE OF GROWING IN GLUTAMINE-FREE MEDIUM

Title:

GLUTAMINE-AUXOTROPHIC HUMAN CELL CAPABLE OF PRODUCING PROTEIN AND CAPABLE OF GROWING IN GLUTAMINE-FREE MEDIUM

Document Type and Number:

Japanese Patent JP2011224010

Kind Code:

A

Abstract:

To provide a novel glutamine-auxotrophic human cell capable of producing a protein and capable of growing in a glutamine-free medium.

The glutamine-auxotrophic human cell transfected with an exogenous DNA sequence encoding a protein or an exogenous DNA sequence capable of altering the expression of an endogenous gene encoding a protein and an exogenous DNA sequence encoding a glutamine synthetase, wherein these exogenous DNA sequences are located on one or more DNA construct, and the transfected cell is capable of producing the protein and capable of growing in the glutamine-free medium.

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Inventors:

BIRCH JOHN
BORASTON ROBERT CHARLES
SHAW MARTYN

Application Number:

JP2011123661A

Publication Date:

November 10, 2011

Filing Date:

June 01, 2011

Export Citation:

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Assignee:

LONZA BIOLOGICS PLC

International Classes:

C12N5/10; C12N15/09; C07K14/505; C12N5/09; C12N5/22; C12N9/00; C12N15/85; C12P21/02

Domestic Patent References:

JPS63502955A	1988-11-02
JPH03500006A	1991-01-10
JP2001511342A	2001-08-14

Other References:

JPN6013020349; Ｂｉｏｔｅｃｈｎｏｌ．Ｂｉｏｅｎｇ．Ｖｏｌ．６８, 2000, ｐ．６３７-６４６

Attorney, Agent or Firm:

Kurata Masatoshi
Satoshi Kono
Makoto Nakamura
Yoshihiro Fukuhara
Takashi Mine
Toshio Shirane
Sadao Muramatsu

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