Title:
核酸の増幅及び検出／定量の効率を改良するためのヘルパーオリゴヌクレオチド
Document Type and Number:
Japanese Patent JP6999645
Kind Code:
B2
Abstract:
Improved methods for the detection and quantitation of a target nucleic acid in a sample using a non-extending helper oligonucleotide are described. The methods include contacting nucleic acids in a sample with amplification reagents including one or more primers, one or more non-extending helper oligonucleotides, and one or more probes. The non-extending helper oligonucleotide facilitates and increases the target nucleic acid accessibility of one or more of the primers, result in greater accumulation of amplicon production, thereby increasing the efficiency and sensitivity of the amplification assay, including amplification assays for Hepatitis C Virus (HCV), for example, HCV Genotype 5. Kits, articles of manufacture, and reaction mixtures are also provided.
Inventors:
Rochaku Mefta
Application Number:
JP2019505388A
Publication Date:
February 04, 2022
Filing Date:
July 26, 2017
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12Q1/686; C12M1/34; C12Q1/6876; G01N33/53
Domestic Patent References:
| JP2010539956A | ||||
| JP2011522559A |
Foreign References:
| WO2015185655A1 | ||||
| WO2008064687A1 | ||||
| WO2008141651A1 |
Attorney, Agent or Firm:
Aoki Atsushi
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Takahide Sasaki
Shinji Mihashi
Youichi Watanabe
Nakajima Masaru
Ryotaro Takei
Takahide Sasaki