Title:
B型肝炎コア抗原融合タンパク質
Document Type and Number:
Japanese Patent JP4713809
Kind Code:
B2
Abstract:
The hepatitis B virus (HBV) capsid is made up of a single species of protein called the core antigen (HBcAg) which self-assembles into particles. The particles are highly immunogenic and are able to present heterologous epitopes to the immune system when the epitopes are inserted into a surface-exposed region of the particles called the "e1 loop". The structural building blocks of the particles are tightly associated dimers of HBcAg in which the adjacent e1 loops are closely juxtaposed. It is proposed that sequences inserted into the e1 loop are conformationally restrained in the assembled particles when presented in monomeric core protein. The invention seeks to solve this problem by covalently linking core proteins as tandem copies (e.g., as dimers) so that insertions can be made independently in each copy. This is particularly useful for insertion of large sequences into the e1 loop because it allows such sequences to be inserted into just one copy of the core protein per tandem repeat, thereby reducing potential conformational clashes in assembly. Alternatively, a different sequence may be inserted into each e1 loop of a tandem repeat, thus increasing the flexibility of HBcAg particles as an epitope delivery system.
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Inventors:
Gain, annick
Gilbert, Robert
Stuart, David
Lowlands, David
Gilbert, Robert
Stuart, David
Lowlands, David
Application Number:
JP2001575628A
Publication Date:
June 29, 2011
Filing Date:
April 09, 2001
Export Citation:
Assignee:
University of Leads Innovations Limited
International Classes:
C12N15/09; A61K39/00; A61K39/29; A61K39/295; A61K48/00; A61P1/16; A61P31/00; A61P31/12; C07K14/02; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/62; C12P21/02; A61K38/00
Domestic Patent References:
| JP63287487A | ||||
| JP62055095A | ||||
| JP3216186A | ||||
| JP6279500A |
Attorney, Agent or Firm:
Hideto Asamura
Hajime Asamura
Naganuma Akio
Yukihiro Ikeda
Hajime Asamura
Naganuma Akio
Yukihiro Ikeda