Title:
C型肝炎ウイルスアシアロ糖タンパク質
Document Type and Number:
Japanese Patent JP4056306
Kind Code:
B2
Abstract:
Two Hepatitis C Virus envelope proteins (E1 and E2) are expressed without sialylation. Recombinant expression of these proteins in lower eukaryotes, or in mammalian cells in which terminal glycosylation is blocked, results in recombinant proteins which are more similar to native HCV glycoproteins. When isolated by GNA lectin affinity, the E1 and E2 proteins aggregate into virus-like particles. Cells bearing a mannose receptor or asialoglycoprotein receptor are capable of being infected with HCV, and supporting culturing of the virus.
Inventors:
Robert Au Ralston
Frank Marcus
Kent Bee.s-Diam
Barbara A. Jarbus
John A. Hall
Frank Marcus
Kent Bee.s-Diam
Barbara A. Jarbus
John A. Hall
Application Number:
JP2002199317A
Publication Date:
March 05, 2008
Filing Date:
July 08, 2002
Export Citation:
Assignee:
Chiron Corporation
International Classes:
C07K14/18; G01N33/576; A61K31/00; A61K38/00; A61K39/00; A61K39/29; A61P31/00; A61P31/12; A61P37/02; C07K1/16; C07K1/22; C07K9/00; C07K14/00; C07K14/42; C07K16/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/00; C12N15/09; C12N15/51; C12P21/02; G01N33/53; C12R1/91; C12R1/93
Domestic Patent References:
| JP2500880A |
Foreign References:
| WO1990011089A1 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita