Title:
HIGH-AFFINITY TCR PROTEIN AND METHOD
Document Type and Number:
Japanese Patent JP2011101649
Kind Code:
A
Abstract:
To provide a T cell receptor (TCR) that has higher affinity for a ligand than wild type TCR.
These high-affinity TCR is obtained by steps of: mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high-affinity TCR can be used in place of an antibody or single chain antibody.
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Inventors:
KRANZ DAVID M
WITTRUP K DANE
HOLLER PHILLIP D
WITTRUP K DANE
HOLLER PHILLIP D
Application Number:
JP2010282453A
Publication Date:
May 26, 2011
Filing Date:
December 17, 2010
Export Citation:
Assignee:
TRUSTEES OF THE UNIV OF ILLINOIS BOARD OF
International Classes:
C12N15/09; G01N33/53; A61K38/00; A61P1/04; A61P3/10; A61P19/02; A61P25/00; A61P29/00; A61P31/04; A61P31/12; A61P33/02; A61P35/00; A61P37/06; C07K14/725; C07K14/74; C12N5/10; C12Q1/02; G01N33/566; G01N33/58
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita