Title:
HIGH-AFFINITY TCR PROTEIN AND METHOD
Document Type and Number:
Japanese Patent JP2014095722
Kind Code:
A
Abstract:
To provide a T cell receptor (TCR) having higher affinity for a ligand than wild type TCR.
These high-affinity TCRs are obtained by steps of: mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on a surface of the yeast cells; and selecting the cells expressing T cell receptor mutants having higher affinity for a peptide/MHC ligand than wild type T cell receptor protein. The high-affinity TCR can be used in place of an antibody or single chain antibody.
Inventors:
KRANZ DAVID M
WITTRUP K DANE
HOLLER PHILLIP D
WITTRUP K DANE
HOLLER PHILLIP D
Application Number:
JP2014006599A
Publication Date:
May 22, 2014
Filing Date:
January 17, 2014
Export Citation:
Assignee:
TRUSTEES OF THE UNIV OF ILLINOIS BOARD OF
International Classes:
G01N33/53; A61K45/00; A61K47/48; A61P31/04; A61P31/12; A61P33/02; A61P35/00; C07K14/725; C07K14/74; C07K16/28; C12N5/10; C12N15/09; C12Q1/02; C40B30/04; C40B40/08; G01N33/533; G01N33/566; G01N33/58
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita
Previous Patent: IMMUNOGENIC STREPTOCOCCUS PROTEIN
Next Patent: ACOUSTIC MEASURING DEVICE AND ACOUSTIC MEASURING METHOD
Next Patent: ACOUSTIC MEASURING DEVICE AND ACOUSTIC MEASURING METHOD