Title:
HIGH CONTENT ANALYSIS AND GENERATION METHOD OF THREE-DIMENSIONAL CELL CULTIVATION MODEL UNDER HIGH RESPIRATION ENVIRONMENT FOR SCREENING
Document Type and Number:
Japanese Patent JP2016146752
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a generation method for three-dimensional cell aggregate with which optical image analysis having excellent robustness in analysis method may be realized.SOLUTION: There is provided a method in which cell aggregate is generated by culturing cells and the cell aggregate is subjected to image analysis, which method is characterized in that: cells are inoculated on medium present on a substantially flat substrate coated with atelocollagen derived from large mammals which is transparent, and having oxygen gas-permeability and liquid impermeability; by continuously supplying cells with air or oxygen containing gas through the substrate, cells are cultured so as to form three-dimensional shape in which the aggregates are cultured in island shape or mountain shape so as to have unevenness, and the aggregates form three-dimensional structures separated with a gap region where substantially cells are absent and arranged in a manner like as separated islands; and by using light penetrating the substrate, on the substrate, the cell aggregates are subjected to photographing analysis.SELECTED DRAWING: None
Inventors:
KANAMUNE JUN
SUMI SHOICHIRO
YOKOGAWA KAZUYUKI
MATSUNAGA HIDEKI
KIM CHENG-WEN
SUMI SHOICHIRO
YOKOGAWA KAZUYUKI
MATSUNAGA HIDEKI
KIM CHENG-WEN
Application Number:
JP2015011826A
Publication Date:
August 18, 2016
Filing Date:
January 23, 2015
Export Citation:
Assignee:
IKKO-ZU:KK
UNIV KYOTO
UNIV KYOTO
International Classes:
C12Q1/02; C12M3/00; C12N5/07; G01N33/48
Domestic Patent References:
| JP2014015415A | 2014-01-30 | |||
| JP2014149235A | 2014-08-21 | |||
| JP2011044016A | 2011-03-03 | |||
| JPH034780A | 1991-01-10 | |||
| JPH08308562A | 1996-11-26 | |||
| JPH07298875A | 1995-11-14 | |||
| JP2014223061A | 2014-12-04 |
Other References:
NISHIZAWA M ET AL.: "Stable immobilization of rat hepatocytes as hemispheroids onto collagen-conjugated poly-dimethylsilo", BIOTECHNOLOGY AND BIOENGINEERING, vol. 99, 6, JPN6018047555, 15 April 2008 (2008-04-15), pages 1472 - 1481, ISSN: 0004060482
KUROSAWA H.ET AL.: "Adult rat hepatocytes cultured on an oxygen-permeable film increases the activity of albumin secreti", CYTOTECHNOLOGY, vol. 36, JPN6018047556, 2001, pages 85 - 92, XP019236694, ISSN: 0003932678, DOI: 10.1023/A:1014028617729
XIAO WJ. ET AL.: "O1-5 Functions and gene expressions of rat hepatocytes under different cultures using oxygen-permeab", ALTERNATIVES TO ANIMAL TESTING AND EXPERIMENTATION, vol. 17, Supplement, JPN6018047557, 31 December 2012 (2012-12-31), pages 148, ISSN: 0003932679
EVENOU F. ET AL.: "Spontaneous formation of highly functional three-dimensional multilayer form human hepatoma Hep G2 c", TISSUE ENGINEERING PART C METHODS, vol. 16, 2, JPN6018047558, April 2010 (2010-04-01), pages 311 - 318, ISSN: 0003932680
NISHIKAWA M. ET AL.: "P-39 Modification poly-dimethylsiloxane (PDMS) surface suitable for the culture of primary rat hepat", ALTERNATIVES TO ANIMAL TESTING AND EXPERIMENTATION, vol. 12, Supplement, JPN6018047559, 31 January 2007 (2007-01-31), pages 141, ISSN: 0003932681
KUROSAWA H.ET AL.: "Adult rat hepatocytes cultured on an oxygen-permeable film increases the activity of albumin secreti", CYTOTECHNOLOGY, vol. 36, JPN6018047556, 2001, pages 85 - 92, XP019236694, ISSN: 0003932678, DOI: 10.1023/A:1014028617729
XIAO WJ. ET AL.: "O1-5 Functions and gene expressions of rat hepatocytes under different cultures using oxygen-permeab", ALTERNATIVES TO ANIMAL TESTING AND EXPERIMENTATION, vol. 17, Supplement, JPN6018047557, 31 December 2012 (2012-12-31), pages 148, ISSN: 0003932679
EVENOU F. ET AL.: "Spontaneous formation of highly functional three-dimensional multilayer form human hepatoma Hep G2 c", TISSUE ENGINEERING PART C METHODS, vol. 16, 2, JPN6018047558, April 2010 (2010-04-01), pages 311 - 318, ISSN: 0003932680
NISHIKAWA M. ET AL.: "P-39 Modification poly-dimethylsiloxane (PDMS) surface suitable for the culture of primary rat hepat", ALTERNATIVES TO ANIMAL TESTING AND EXPERIMENTATION, vol. 12, Supplement, JPN6018047559, 31 January 2007 (2007-01-31), pages 141, ISSN: 0003932681
Attorney, Agent or Firm:
Masato Tsuda
Tetsuji Nakamura
Katsuyuki Tomita
Husband
Ariyoshi Yasuomi
Ryu Maesawa
Tamako Tsutada
Tetsuji Nakamura
Katsuyuki Tomita
Husband
Ariyoshi Yasuomi
Ryu Maesawa
Tamako Tsutada
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