To provide a high sensitivity multiparameter method for rare event analysis.
A sample of blood containing CTCs, or other cells of interest, is stained with fluorescent markers for image analysis and scanned to identify the presence and location within the cartridge of target cells or subcellular elements. A sample containing desired target cells or subcellular elements is then further processed, in part by photobleaching the sample, so that those same targets may be re-analyzed with additional biomarkers conjugated to the same or different fluorochromes by using the same imaging criteria that were used for the initial analysis. Applications with targets such as circulating epithelial cells, circulating tumor cells, circulating endothelial cells, leukocytes, lymphocyte subsets, cells containing an organelle or receptor of interest, cellular debris, disrupted cells and their debris, or any other formed element that might be captured and imaged, are provided.
COUMANS FRANK
GROSS STEVE
KELLY MIKE
| JP2003516129A | 2003-05-13 | |||
| JP2007523626A | 2007-08-23 | |||
| JP2007505626A | 2007-03-15 | |||
| JP2005524833A | 2005-08-18 | |||
| JP2002517183A | 2002-06-18 |
| WO2007053245A2 | 2007-05-10 |
Hiroshi Oshino
Yutaka Nagata
Takafumi Oshima
Chie Fujita