Title:
クルイベロマイセス・マルシアヌス由来の高発現プロモーター
Document Type and Number:
Japanese Patent JP5804378
Kind Code:
B2
Abstract:
The present invention provides a novel high-expression promoter, i.e., a GAL1 promoter, derived from Kluyveromyces marxianus, a recombinant polynucleotide containing the high-expression promoter, a vector containing the recombinant polynucleotide, a transformant obtained by introducing the recombinant polynucleotide or the vector into yeast, a method for highly expressing a target gene using the transformant and a method for producing a target gene product using the transformant. The present invention is characterized by use of the high-expression promoter of the present invention.
Inventors:
Rinji Akada
Shoji Hoshida
Masamitsu Ide
Shoji Hoshida
Masamitsu Ide
Application Number:
JP2011553749A
Publication Date:
November 04, 2015
Filing Date:
February 07, 2011
Export Citation:
Assignee:
Yamaguchi University
International Classes:
C12N15/09; C12N1/19; C12P21/00
Domestic Patent References:
| JPS62253384A | 1987-11-05 |
Other References:
JPN6011011501; ＷＩＣＫＥＳ， Ｂｒｉａｎ Ｌ． ａｎｄ ＥＤＭＡＮ Ｊｅｆｆｒｅｙ Ｃ．: 'Ｔｈｅ Ｃｒｙｐｔｏｃｏｃｃｕｓ ｎｅｏｆｏｒｍａｎｓ ＧＡＬ７ ｇｅｎｅ ａｎｄ ｉｔｓ ｕｓｅ ａｓ ａｎ ｉｎｄｕｃｉｂｌｅ ｐｒｏｍｏｔｅｒ．' Ｍｏｌ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． １６， Ｎｏ． ６, 1995, ｐ． １０９９-１１０９
JPN6011011502; ＮＯＮＫＬＡＮＧ， Ｓａｎｏｍ ｅｔ ａｌ．: 'Ｈｉｇｈ-ｔｅｍｐｅｒａｔｕｒｅ ｅｔｈａｎｏｌ ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ｗｉｔｈ ｌｉｎｅａｒ ＤＮＡ ｉｎ ｔｈｅ ｔｈｅｒｍｏｔｏｌｅｒａｎｔ ｙｅａｓｔ' Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． ７４， Ｎｏ． ２４, 2008, ｐ． ７５１４-７５２１
JPN6011011503; ＷＥＢＳＴＥＲ， Ｔｈｏｍａｓ Ｄ． ａｎｄ ＤＩＣＫＳＯＮ， Ｒｏｂｅｒｔ Ｃ．: 'Ｎｕｃｌｅｏｔｉｄｅ ｓｅｑｕｅｎｃｅ ｏｆ ｔｈｅ ｇａｌａｃｔｏｓｅ ｇｅｎｅ ｃｌｕｓｔｅｒ ｏｆ Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｌａｃｔｉｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． １６， Ｎｏ． １６, 1988, ｐ． ８１９２-８１９４
JPN6011011504; ＳＯＵＣＩＥＴ， Ｊｅａｎ-Ｌｕｃ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １． Ａ ｓｅｔ ｏｆ ｙｅａｓｔ ｓｐｅｃｉｅｓ ｆｏｒ ｍｏｌｅｃｕｌａｒ ｅｖｏｌｕｔｉｏ' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ３-１２
JPN6011011505; ＬＬＯＲＥＮＴＥ， Ｂｅｒｔｒａｎｄ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １２． Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｍａｒｘｉａｎｕｓ ｖａｒ． ｍａｒｘｉａｎｕｓ．' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ７１-７５
JPN6011011485; ＤＮＡ Ｄａｔａ Ｂａｎｋ ｏｆ Ｊａｐａｎ． ［ｏｎｌｉｎｅ］． , 2005
JPN6011011499; ＰＡＲＫ， Ｓｕｎ Ｍｅｅ ｅｔ ａｌ．: 'Ｇａｌａｃｔｏｓｅ-ｉｎｄｕｃｉｂｌｅ ｅｘｐｒｅｓｓｉｏｎ ｓｙｓｔｅｍｓ ｉｎ Ｃａｎｄｉｄａ ｍａｌｔｏｓａ ｕｓｉｎｇ ｐｒｏｍｏｔｅｒｓ ｏｆ ｎｅｗｌｙ-ｉｓｏｌａｔｅｄ ＧＡＬ１ ａｎｄ' Ｙｅａｓｔ． Ｖｏｌ． １３, 1997, ｐ． ２１-２９
JPN6011011501; ＷＩＣＫＥＳ， Ｂｒｉａｎ Ｌ． ａｎｄ ＥＤＭＡＮ Ｊｅｆｆｒｅｙ Ｃ．: 'Ｔｈｅ Ｃｒｙｐｔｏｃｏｃｃｕｓ ｎｅｏｆｏｒｍａｎｓ ＧＡＬ７ ｇｅｎｅ ａｎｄ ｉｔｓ ｕｓｅ ａｓ ａｎ ｉｎｄｕｃｉｂｌｅ ｐｒｏｍｏｔｅｒ．' Ｍｏｌ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． １６， Ｎｏ． ６, 1995, ｐ． １０９９-１１０９
JPN6011011502; ＮＯＮＫＬＡＮＧ， Ｓａｎｏｍ ｅｔ ａｌ．: 'Ｈｉｇｈ-ｔｅｍｐｅｒａｔｕｒｅ ｅｔｈａｎｏｌ ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ｗｉｔｈ ｌｉｎｅａｒ ＤＮＡ ｉｎ ｔｈｅ ｔｈｅｒｍｏｔｏｌｅｒａｎｔ ｙｅａｓｔ' Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． ７４， Ｎｏ． ２４, 2008, ｐ． ７５１４-７５２１
JPN6011011503; ＷＥＢＳＴＥＲ， Ｔｈｏｍａｓ Ｄ． ａｎｄ ＤＩＣＫＳＯＮ， Ｒｏｂｅｒｔ Ｃ．: 'Ｎｕｃｌｅｏｔｉｄｅ ｓｅｑｕｅｎｃｅ ｏｆ ｔｈｅ ｇａｌａｃｔｏｓｅ ｇｅｎｅ ｃｌｕｓｔｅｒ ｏｆ Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｌａｃｔｉｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． １６， Ｎｏ． １６, 1988, ｐ． ８１９２-８１９４
JPN6011011504; ＳＯＵＣＩＥＴ， Ｊｅａｎ-Ｌｕｃ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １． Ａ ｓｅｔ ｏｆ ｙｅａｓｔ ｓｐｅｃｉｅｓ ｆｏｒ ｍｏｌｅｃｕｌａｒ ｅｖｏｌｕｔｉｏ' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ３-１２
JPN6011011505; ＬＬＯＲＥＮＴＥ， Ｂｅｒｔｒａｎｄ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １２． Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｍａｒｘｉａｎｕｓ ｖａｒ． ｍａｒｘｉａｎｕｓ．' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ７１-７５
JPN6011011502; ＮＯＮＫＬＡＮＧ， Ｓａｎｏｍ ｅｔ ａｌ．: 'Ｈｉｇｈ-ｔｅｍｐｅｒａｔｕｒｅ ｅｔｈａｎｏｌ ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ｗｉｔｈ ｌｉｎｅａｒ ＤＮＡ ｉｎ ｔｈｅ ｔｈｅｒｍｏｔｏｌｅｒａｎｔ ｙｅａｓｔ' Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． ７４， Ｎｏ． ２４, 2008, ｐ． ７５１４-７５２１
JPN6011011503; ＷＥＢＳＴＥＲ， Ｔｈｏｍａｓ Ｄ． ａｎｄ ＤＩＣＫＳＯＮ， Ｒｏｂｅｒｔ Ｃ．: 'Ｎｕｃｌｅｏｔｉｄｅ ｓｅｑｕｅｎｃｅ ｏｆ ｔｈｅ ｇａｌａｃｔｏｓｅ ｇｅｎｅ ｃｌｕｓｔｅｒ ｏｆ Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｌａｃｔｉｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． １６， Ｎｏ． １６, 1988, ｐ． ８１９２-８１９４
JPN6011011504; ＳＯＵＣＩＥＴ， Ｊｅａｎ-Ｌｕｃ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １． Ａ ｓｅｔ ｏｆ ｙｅａｓｔ ｓｐｅｃｉｅｓ ｆｏｒ ｍｏｌｅｃｕｌａｒ ｅｖｏｌｕｔｉｏ' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ３-１２
JPN6011011505; ＬＬＯＲＥＮＴＥ， Ｂｅｒｔｒａｎｄ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １２． Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｍａｒｘｉａｎｕｓ ｖａｒ． ｍａｒｘｉａｎｕｓ．' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ７１-７５
JPN6011011485; ＤＮＡ Ｄａｔａ Ｂａｎｋ ｏｆ Ｊａｐａｎ． ［ｏｎｌｉｎｅ］． , 2005
JPN6011011499; ＰＡＲＫ， Ｓｕｎ Ｍｅｅ ｅｔ ａｌ．: 'Ｇａｌａｃｔｏｓｅ-ｉｎｄｕｃｉｂｌｅ ｅｘｐｒｅｓｓｉｏｎ ｓｙｓｔｅｍｓ ｉｎ Ｃａｎｄｉｄａ ｍａｌｔｏｓａ ｕｓｉｎｇ ｐｒｏｍｏｔｅｒｓ ｏｆ ｎｅｗｌｙ-ｉｓｏｌａｔｅｄ ＧＡＬ１ ａｎｄ' Ｙｅａｓｔ． Ｖｏｌ． １３, 1997, ｐ． ２１-２９
JPN6011011501; ＷＩＣＫＥＳ， Ｂｒｉａｎ Ｌ． ａｎｄ ＥＤＭＡＮ Ｊｅｆｆｒｅｙ Ｃ．: 'Ｔｈｅ Ｃｒｙｐｔｏｃｏｃｃｕｓ ｎｅｏｆｏｒｍａｎｓ ＧＡＬ７ ｇｅｎｅ ａｎｄ ｉｔｓ ｕｓｅ ａｓ ａｎ ｉｎｄｕｃｉｂｌｅ ｐｒｏｍｏｔｅｒ．' Ｍｏｌ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． １６， Ｎｏ． ６, 1995, ｐ． １０９９-１１０９
JPN6011011502; ＮＯＮＫＬＡＮＧ， Ｓａｎｏｍ ｅｔ ａｌ．: 'Ｈｉｇｈ-ｔｅｍｐｅｒａｔｕｒｅ ｅｔｈａｎｏｌ ｆｅｒｍｅｎｔａｔｉｏｎ ａｎｄ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ｗｉｔｈ ｌｉｎｅａｒ ＤＮＡ ｉｎ ｔｈｅ ｔｈｅｒｍｏｔｏｌｅｒａｎｔ ｙｅａｓｔ' Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ． Ｖｏｌ． ７４， Ｎｏ． ２４, 2008, ｐ． ７５１４-７５２１
JPN6011011503; ＷＥＢＳＴＥＲ， Ｔｈｏｍａｓ Ｄ． ａｎｄ ＤＩＣＫＳＯＮ， Ｒｏｂｅｒｔ Ｃ．: 'Ｎｕｃｌｅｏｔｉｄｅ ｓｅｑｕｅｎｃｅ ｏｆ ｔｈｅ ｇａｌａｃｔｏｓｅ ｇｅｎｅ ｃｌｕｓｔｅｒ ｏｆ Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｌａｃｔｉｓ．' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． １６， Ｎｏ． １６, 1988, ｐ． ８１９２-８１９４
JPN6011011504; ＳＯＵＣＩＥＴ， Ｊｅａｎ-Ｌｕｃ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １． Ａ ｓｅｔ ｏｆ ｙｅａｓｔ ｓｐｅｃｉｅｓ ｆｏｒ ｍｏｌｅｃｕｌａｒ ｅｖｏｌｕｔｉｏ' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ３-１２
JPN6011011505; ＬＬＯＲＥＮＴＥ， Ｂｅｒｔｒａｎｄ ｅｔ ａｌ．: 'Ｇｅｎｏｍｉｃ ｅｘｐｌｏｒａｔｉｏｎ ｏｆ ｔｈｅ ｈｅｍｉａｓｃｏｍｙｃｅｔｏｕｓ ｙｅａｓｔｓ： １２． Ｋｌｕｙｖｅｒｏｍｙｃｅｓ ｍａｒｘｉａｎｕｓ ｖａｒ． ｍａｒｘｉａｎｕｓ．' ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４８７, 2000, ｐ． ７１-７５
Attorney, Agent or Firm:
Masaki Hirota