Title:
突然変異グルコース-6-リン酸デヒドロゲナーゼを利用するホモジナスイムノアッセイ
Document Type and Number:
Japanese Patent JP4223546
Kind Code:
B2
Abstract:
The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
Inventors:
Jakobowitz, Edward Bee.
Siren, Joyel.
Levy, Mark Jay.
Goodman, Thomas C.
Becker, Martin Jay.
Ullman, Edwin F.
Caldwell, Robert M.
Bot, Richard Earl.
Burnet, Christopher Charles
Siren, Joyel.
Levy, Mark Jay.
Goodman, Thomas C.
Becker, Martin Jay.
Ullman, Edwin F.
Caldwell, Robert M.
Bot, Richard Earl.
Burnet, Christopher Charles
Application Number:
JP52322794A
Publication Date:
February 12, 2009
Filing Date:
April 07, 1994
Export Citation:
Assignee:
Dade Behring Marburg Gezershyaft Mitt Besilenktel Haftung
Genenco International Inc.
Genenco International Inc.
International Classes:
C12N9/04; G01N33/542; C12N15/09; C12Q1/28; C12Q1/32; G01N33/535; G01N33/569; G01N33/58; G01N33/78; G01N33/94; C12R1/19
Domestic Patent References:
| JP389163A | ||||
| JP62235566A |
Other References:
Protein Science,1992, Vol.1, No.3, pp.329-334
Biosens Bioelectron,1993 Jan., Vol.8, No.9/10, pp.415-419
Biosens Bioelectron,1993 Jan., Vol.8, No.9/10, pp.415-419
Attorney, Agent or Firm:
Takashi Ishida
Yoshio Yoshio
Masaya Nishiyama
Yoshio Yoshio
Masaya Nishiyama