Title:
スタッガード(staggered)ライゲーションを使用して核酸配列を増幅する方法
Document Type and Number:
Japanese Patent JP4599483
Kind Code:
B2
Abstract:
Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules are prepared by: providing a single stranded cDNA molecule having 5' and 3' ends; attaching an oligodeoxynucleotide tail to the 3' end of said single stranded cDNA molecule; providing a double stranded RNA polymerase promoter having a sense strand and antisense strand, wherein the sense strand comprises a single stranded 3' overhang comprising a sequence complementary to said oligodeoxynucleotide tail; annealing said double stranded RNA polymerase promoter to said oligodeoxynucleotide tail by complementary base pairing with said 3' overhang sequence; ligating the 5' end of the antisense strand of said double stranded RNA polymerase promoter to the 3' end of said oligodeoxynucleotide tail; and initiating RNA transcription using an RNA polymerase which recognizes said double stranded promoter, thus producing a sense RNA molecule (sRNA).
Inventors:
Getz, Robert Sea
Shwarm, jamie
Shwarm, jamie
Application Number:
JP2006532858A
Publication Date:
December 15, 2010
Filing Date:
May 07, 2004
Export Citation:
Assignee:
Jennifer erucy
International Classes:
C12N15/09; C12P19/34; C12Q1/68; G01N21/78; C12N
Domestic Patent References:
| JP2002360249A | ||||
| JP5500012A |
Attorney, Agent or Firm:
Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo
Hidefumi Kawamura
Naomi Yoshida
Ayako Nakamura
Fukagawa Eri
Satoshi Morimoto
Kyoko Tsunoda
Hirose Miki
Arihara Koichi
Matsushima Tetsuo
Hidefumi Kawamura
Naomi Yoshida
Ayako Nakamura
Fukagawa Eri
Satoshi Morimoto
Kyoko Tsunoda
Hirose Miki