Title:
糖タンパク質を定量的プロテオ-ム分析する方法
Document Type and Number:
Japanese Patent JP4583168
Kind Code:
B2
Abstract:
The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.
Inventors:
Abersold, Rudolf H.
Zan, Hui
Zan, Hui
Application Number:
JP2004509709A
Publication Date:
November 17, 2010
Filing Date:
June 03, 2003
Export Citation:
Assignee:
The Institute for Systems Biology
International Classes:
G01N33/68; C12Q1/34; C12Q1/37; G01N27/62
Other References:
Minghui Geng et al.,Signature-peptide approach to detecting proteins in complex mixtures,Journal of Chromatography A,2000年,Volume 870, Issues 1-2,295-313
Xiaohong Qian et al.,Direct Analysis of the Products of Sequential Cleavages of Peptides and Proteins Affinity-Bound to Immobilized Metal Ion Beads by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Analytical Biochemistry,1999年,Volume 274, Issue 2,,174-180
O'Shannessy DJ et al.,Site-directed immobilization of glycoproteins on hydrazide-containing solid supports,iotechnology and Applied Biochemistry,1987年,Vol. 9,488-496
Anne Dell et al.,Glycoprotein Structure Determination by Mass Spectrometry,SCIENCE,2001年,VOL. 291,2351-2356
Franz-Georg Hanisch et al.,Glycoprotein Identification and Localization of O-Glycosylation Sites by Mass Spectrometric Analysis of Deglycosylated/Alkylaminylated Peptide Fragments,Analytical Biochemistry,2001年,Volume 290, Issue 1,47-59
Xiaohong Qian et al.,Direct Analysis of the Products of Sequential Cleavages of Peptides and Proteins Affinity-Bound to Immobilized Metal Ion Beads by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, Analytical Biochemistry,1999年,Volume 274, Issue 2,,174-180
O'Shannessy DJ et al.,Site-directed immobilization of glycoproteins on hydrazide-containing solid supports,iotechnology and Applied Biochemistry,1987年,Vol. 9,488-496
Anne Dell et al.,Glycoprotein Structure Determination by Mass Spectrometry,SCIENCE,2001年,VOL. 291,2351-2356
Franz-Georg Hanisch et al.,Glycoprotein Identification and Localization of O-Glycosylation Sites by Mass Spectrometric Analysis of Deglycosylated/Alkylaminylated Peptide Fragments,Analytical Biochemistry,2001年,Volume 290, Issue 1,47-59
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita