Title:
DNA一本鎖切断を検出する方法
Document Type and Number:
Japanese Patent JP4558710
Kind Code:
B2
Abstract:
A method of detecting increased levels of DNA single strand breaks in a eukaryotic cell sample, comprising the steps of: (a) contacting a eukaryotic cell sample to a water-soluble tetrazolium salt under conditions in which said tetrazolium salt is converted to a formazan dye in said cell sample in the presence of NADH or NADPH; and then (b) detecting the presence of the formazan dye in said cell sample, with decreased levels of the formazan dye indicating increased levels of DNA single strand breaks in the eukaryotic cell sample.
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Inventors:
Jun Nakamura
Swaenberg, James A.
Swaenberg, James A.
Application Number:
JP2006500778A
Publication Date:
October 06, 2010
Filing Date:
January 06, 2004
Export Citation:
Assignee:
University of North Carolina at Chapel Hill
International Classes:
C12Q1/68; C12Q1/02; G01N21/75; G01N21/78; G01N33/15; G01N33/50
Domestic Patent References:
| JP63276499A | ||||
| JP9286784A |
Foreign References:
| US5185450 |
Other References:
POGREBNIAK, A. et al.,"Inhibitors of PARP protect leukemic cells from delayed cell death by three different mechanisms.",BLOOD,2001年11月16日,Vol.98, No.11, Part 2,P.140b,Abstract#4227欄
CANTONI, O. et al.,"Hydrogen peroxide insult in cultured mammalian cells: ralationships between DNA single-strand breakage, poly(ADP-ribose) metabolism and cell killing.",BIOCHIM. BIOPHYS. ACTA,1989年,Vol.1014, No.1,P.1-7
CARDINAL, J.W. et al.,"Poly(ADP-ribose)polymerase activation determines strain sensitivity to streptozotocin-induced beta cell death in inbred mice.",J. MOL. ENDOCRINOL.,1999年 2月,Vol.22, No.1,P.65-70
BERRIDGE, M.V. and TAN, A.S.,"Trans-plasma membrane electron transport: a cellular assay for NADH- and NADPH-oxidase based on extracellular, superoxide-mediated reduction of the sulfonated tetrazolium salt WST-1.",PROTOPLASMA,1998年 6月,Vol.205,P.74-82
NAKAMURA, J. et al.,"Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time.",NUCLEIC ACIDS RESEARCH,2003年 9月,Vol.31, No.17,e104, P.1-7
TICE, R.R. et al.,"Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing.",ENVIRON. MOL. MUTAGEN.,2000年,Vol.35, No.3,P.206-221
CANTONI, O. et al.,"Hydrogen peroxide insult in cultured mammalian cells: ralationships between DNA single-strand breakage, poly(ADP-ribose) metabolism and cell killing.",BIOCHIM. BIOPHYS. ACTA,1989年,Vol.1014, No.1,P.1-7
CARDINAL, J.W. et al.,"Poly(ADP-ribose)polymerase activation determines strain sensitivity to streptozotocin-induced beta cell death in inbred mice.",J. MOL. ENDOCRINOL.,1999年 2月,Vol.22, No.1,P.65-70
BERRIDGE, M.V. and TAN, A.S.,"Trans-plasma membrane electron transport: a cellular assay for NADH- and NADPH-oxidase based on extracellular, superoxide-mediated reduction of the sulfonated tetrazolium salt WST-1.",PROTOPLASMA,1998年 6月,Vol.205,P.74-82
NAKAMURA, J. et al.,"Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time.",NUCLEIC ACIDS RESEARCH,2003年 9月,Vol.31, No.17,e104, P.1-7
TICE, R.R. et al.,"Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing.",ENVIRON. MOL. MUTAGEN.,2000年,Vol.35, No.3,P.206-221
Attorney, Agent or Firm:
Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo
Arihara Koichi
Matsushima Tetsuo