Title:
乳汁中のストレプトコッカス属の菌を検出する方法
Document Type and Number:
Japanese Patent JP6370807
Kind Code:
B2
Abstract:
The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of various Streptococcus bacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is a Streptococcus bacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing a Streptococcus bacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse a Streptococcus bacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and ²-N-acetylglucosaminidase.
Inventors:
Takamitsu Utsumi
Hiroshi Maehana
Kenji Matsuyama
Hiroshi Maehana
Kenji Matsuyama
Application Number:
JP2015553592A
Publication Date:
August 08, 2018
Filing Date:
December 17, 2014
Export Citation:
Assignee:
ASAHI KASEI KABUSHIKI KAISHA
International Classes:
C12N1/06; C12M1/34; C12M1/40; C12N9/24; C12N9/36; C12N9/48; C12Q1/14; C12Q1/34; C12Q1/37; G01N33/48; G01N33/532; G01N33/543; G01N33/569
Domestic Patent References:
| JP3501653A | ||||
| JP2012122921A | ||||
| JP2003171291A | ||||
| JP9227392A | ||||
| JP2012524548A | ||||
| JP2011512861A | ||||
| JP2006504619A |
Foreign References:
| WO2007013452A1 |
Other References:
Indian J. Microbiol.,2012年,vol.52 no.2,pp.153-159
J. Dairy Sci.,2005年,vol.88,pp.3510-3518
Journal of Microbiological Methods,2005年,Vol.61,pp.251-260
Food Research International,2009年,vol.42,pp.893-898
Biochemical and Biophysical Research Communications,1989年,Vol.158, No.1,pp.251-256
Journal of Dairy Research,1984年,Vol.51,pp.11-16
J. Dairy Sci.,2005年,vol.88,pp.3510-3518
Journal of Microbiological Methods,2005年,Vol.61,pp.251-260
Food Research International,2009年,vol.42,pp.893-898
Biochemical and Biophysical Research Communications,1989年,Vol.158, No.1,pp.251-256
Journal of Dairy Research,1984年,Vol.51,pp.11-16
Attorney, Agent or Firm:
Patent Service Corporation Patent Office Sykes